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Acid Fast Stain Table 1: The cells of mycobacterium measured were reddish-purple and spherical in shape with a diameter of 0. Endospore Stain Table 2: Spores showed up as green-colored coccobacilli, which are located in the center of the cells.
Simple Stain Table 3: Negative Stain Table 4: Flagella Stain Table 5: Gram Stain Table 6: Escherichia coli, a Gram-negative bacteria appeared as a reddish-pink spherical shaped, clumped together with a cell diameter of 0.
Acid-Fast Stain Acid-fast microorganisms have mycolic acids present in their cell walls which assists with attaching a stain into these cell that will be resistant to decolorization.
There are two different kinds of acid-fast staining procedures: The ZN method requires the use of heat in the staining process while the K method does not.
The purpose of heating during the staining procedure of the ZN method was to melt the wax within the cell wall to enable the stain enter into the cell.
Carbolfuchsin was the primary stain used while methylene blue was the counterstain used after decolorization. This indicated that mycobacterium had mycolic acids present within their cell walls since the stain carbolfuchsin had successfully entered these cells. Endospore Stain The endospore stain Malachite green is used to dye spores produced by bacteria.
When an older bacteria culture undergoes nutrient depletion, its response will include production of spores. Steaming the bacterial emulsion on the slide while utilizing endospore staining procedure forced Malachite green to enter the spore.
Malachite green will only color the spores present on the slide, since this dye has lower affinity for cellular materials. Vegetative cells and spore mother cells other than the spores themselves will be colored by the counterstain safranin.
This organism had green dots located in the center of the cell which indicated that had nutrient depletion in the Bacillus cereus had caused it to produce coccobacillus shaped spores in order to survive.
Simple Stain Simple stains are used to color bacteria which have been heat-fixed to the slide. The heat-fixing step is required to kill bacteria and make them more visible after staining.
These three microorganisms appeared blue because of color of the dye, proving that the negative charges on their surfaces readily absorbed this positively charged basic stain.
Negative Stain Negative stain uses negatively charged dye which can repel the negatively charged surfaces of bacteria resulting in the appearance of unstained bacteria cells with dark backgrounds.
Both microorganisms remained unstained with dark backgrounds after staining with nigrosin.
This indicates that both Micrcoccus luteus and Bacillus megaterium have negatively charged surfaces which repel the negative charged nigrosine stain.
Flagella Stain Flagella in bacteria are too thin to be observed using regular stains. Mordant can be used to overcome this problem so that flagella will appear thick enough to be observed. Several flagella emerging on one end of the cell have lophotichous arrangements, while flagella emerging from around the cell have peritrichous arrangements.
Gram Stain Gram stain is used to differentiate Gram-positive bacteria from Gram-negative bacteria. Gram- positive bacteria have a thicker peptidoglycan which makes up their cell walls. Meanwhile, Gram-negative bacteria have thinner peptidoglycan which makes up their cell walls but have greater lipid content since these bacteria have an outer membrane.
Therefore, it is determined to be a Gram-negative bacteria. The decolorizer extracted the lipid from outer membrane of Gram-negative bacteria washed off the crystal violet- iodine complex. Safranin then was used to counterstain E. This indicates that both microorganisms were Gram-positive bacteria which have thicker peptidoglycans in their cell walls that trap the crystal violet-iodine complex more effectively, making them resistant to the decolorizer.
Leboffe M, Pierce BE. In Microbiology Laboratory Theory and Application, 3rd ed.In a manner quite similar to the Gram stain, the acid-fast stain differentiates an important group of bacteria, the mycobacteria, on the basis of lipid morphology after fixation and staining.
The slide is then stained with crystal violet dye, which is Staphylococcus aureus, Gram positive coccus 1 plate/table Neisseria flava. A bacterial wound culture is a test that detects and identifies bacteria that cause infections in a attheheels.com wound may become infected with a variety of bacteria.
A culture helps to determine whether a wound has become infected, which type(s) of bacteria are causing the infection, and which antibiotic would best treat the infection and help heal the wound. Test Description. After a diagnosis of metastatic breast cancer has been made, Her-2/neu testing may be used as a prognostic marker to help determine how aggressive the breast cancer tumor is likely to be.
THE VIRTUAL EDGE: Lab 3 Bacterial Staining Techniques II Acidfast Stain: Background and Introduction Mycobacterium and many Nocardia species are called acid-fast because during an acid-fast staining procedure they retain the primary dye carbol fuchsin despite decolorization with .
Stab one with Pseudomonas aeruginosa and the other with Staphylococcus aureus. Stab the bacterium about 1/2 - 3/4 of an inch into the agar, taking care not to tilt or twist the loop so that the loop comes up through the same cut as it went down.
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